Shown below are steps in making a transgenic plant.
1)Expose plant cells to Agrobacterium tumefaciens
2)Place plant cells on selective medium
3)Transform the recombinant plasmid into Agrobacterium tumefaciens
4)Insert gene of interest into Ti plasmid
5)Place plant cells on growth medium with plant hormones
What is the correct order of these steps?

Question

Quizplus · Accepted Answer

The correct order for making a transgenic plant using Agrobacterium tumefaciens starts with inserting the gene of interest into the Ti plasmid (4), followed by transforming the recombinant plasmid into Agrobacterium tumefaciens (3). The next step involves exposing plant cells to the transformed Agrobacterium tumefaciens (1), then placing the plant cells on selective medium to select for successfully transformed cells (2), and finally placing the plant cells on growth medium with plant hormones to encourage the growth of a new plant (5).

Shown Below Are Steps in Making a Transgenic Plant