In recombinant DNA technique,what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

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DNA ligase is the enzyme that seals the sticky ends of genes into plasmids or chromosomes by creating a phosphodiester bond between the sugar and phosphate backbone of adjacent nucleotides. DNA polymerase I and II are involved in DNA replication and repair, while DNA helicase is responsible for unwinding DNA strands. Primase is involved in the initiation of DNA replication by synthesizing RNA primers.

In Recombinant DNA Technique,what Enzyme Is Needed to Seal the Sticky