Which of the following are necessary for DNA replication in vitro?
A)RNA,helicase,primers,DNA polymerase
B)Okazaki fragments,helicase,DNA polymerase
C)Template DNA,DNA polymerase,four dNTPs,primers,magnesium ions
D)Template DNA,four dNTPs,magnesium ions
E)DNA can't replicate in vitro

Question

Quizplus · Accepted Answer

To replicate DNA in vitro, we need template DNA to build on, DNA polymerase to catalyze the formation of phosphodiester bonds between the nucleotides, four deoxynucleoside triphosphates (dNTPs) to serve as the building blocks for the new DNA strand, primers that initiate DNA synthesis, and magnesium ions that assist in the catalytic activity of DNA polymerase. Therefore, choice C includes all the necessary components for DNA replication in vitro.

Which of the Following Are Necessary for DNA Replication in Vitro