In addition to restriction enzymes,which of the following enzymes is required to insert a fragment of DNA into a cloning vector?
A)DNA polymerase
B)DNA ligase
C)RNA polymerase
D)Reverse transcriptase
E)Topoisomerase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme that is required to seal the nick between the vector and the DNA fragment, forming a stable recombinant molecule. DNA polymerase is important for DNA synthesis, but it is not directly involved in vector insertion. RNA polymerase is used for transcription, not cloning. Reverse transcriptase is used for converting RNA into DNA (reverse transcription), but it is not directly involved in the process of cloning. Topoisomerase is important for relieving the tension created by the opening of the double helix during replication or transcription, but it is not involved in vector insertion.

In Addition to Restriction Enzymes,which of the Following Enzymes Is