Successful insertion of a DNA fragment into the polylinker region of pBluescript II is detected by
A)production of the enzyme β-galactosidase.
B)absence of the enzyme β-galactosidase.
C)the increased size of the plasmid.
D)autoradiography.
E)None of these

Question

Quizplus · Accepted Answer

The successful insertion of a DNA fragment into the polylinker region of pBluescript II disrupts the lacZ gene, which encodes the enzyme β-galactosidase. Therefore, colonies with successful insertions will not produce β-galactosidase, leading to the absence of blue color in the presence of X-gal, indicating successful cloning.

Successful Insertion of a DNA Fragment into the Polylinker Region