What is the advantage of adding SDS to gel electrophoresis?
A) SDS colors the proteins for visualization.
B) SDS reduces disulfide bonds.
C) SDS allows proteins to be separated on the basis of approximate mass.
D) None of the answers is correct.
E) All of the answers are correct.

Question

Quizplus · Accepted Answer

SDS (sodium dodecyl sulfate) is a detergent that denatures proteins and gives them a uniform negative charge. This allows proteins to be separated by size during gel electrophoresis, as the uniform charge ensures that the migration speed through the gel is determined by the size of the protein, not its charge.

What Is the Advantage of Adding SDS to Gel Electrophoresis