Each cycle in PCR includes
A) DNA synthesis.
B) hybridization of primers.
C) strand separation.
D) restriction.
E) DNA synthesis, hybridization of primers, and strand separation.

Question

Quizplus · Accepted Answer

Each cycle in PCR (Polymerase Chain Reaction) involves three main steps: DNA synthesis, where new strands of DNA are synthesized; hybridization of primers, where short DNA sequences (primers) bind to the DNA template; and strand separation, where the double-stranded DNA is denatured into single strands to allow the cycle to repeat. Restriction is not a part of the PCR cycle.

Each Cycle in PCR Includes