Which of the following techniques allows investigators to test the role of individual amino acids in the determination of enzyme structure/function relationships even if the investigated amino acid is not present in the active site?
A) stopped-flow assay
B) use of mechanism based inactivators
C) use of methylating agents
D) site-directed mutagenesis
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

Site-directed mutagenesis is a molecular biology method that allows for the creation of specific, targeted changes in a gene, thereby altering the amino acid sequence of the encoded protein. This technique can be used to investigate the role of individual amino acids in enzyme structure and function, including those not located in the active site, by observing the effects of these changes on the enzyme's activity, stability, and other properties.

Which of the Following Techniques Allows Investigators to Test the Role