Changes in ATCase conformation were detected by crystallizing the enzyme in the presence of PALA (N-(phosphonacetyl)-L-aspartate). What is PALA?
A) a radioactive tag that binds to the subunits
B) a substrate analog that resembles the transition state
C) a fluorescent allosteric inhibitor analog
D) All of the answers are correct.
E) None of the answers is correct.

Question

Quizplus · Accepted Answer

PALA is a substrate analog that resembles the transition state of the reaction catalyzed by aspartate transcarbamoylase (ATCase), used to study the enzyme's conformational changes upon binding.

Changes in ATCase Conformation Were Detected by Crystallizing the Enzyme