Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

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DNA sequencing, especially when referring to Sanger sequencing or dideoxynucleotide sequencing, can indeed be performed with a single primer (either forward or reverse) because the method relies on the generation of variously sized fragments from a single starting point, which are then separated by size. This process does not require the simultaneous use of both forward and reverse primers as in typical PCR amplification processes.

Polymerase Chain Reaction (PCR) Can Be Used to Perform DNA