The standard method for detecting antinuclear antibodies (in certain autoimmune diseases)is:

Question

Quizplus · Accepted Answer

The standard method for detecting antinuclear antibodies is indirect immunofluorescence. This involves incubating a patient's serum with a substrate that contains fixed cells, which are then washed and exposed to a fluorescently-labeled secondary antibody. The presence of antinuclear antibodies can be detected by the characteristic pattern of fluorescence that appears under a microscope. Direct immunofluorescence involves using fluorescently-labeled primary antibodies to directly detect antigens on tissue samples. However, this is not the standard method for detecting antinuclear antibodies in autoimmune diseases, so option A is incorrect. Fluorescence polarization is a technique used in biochemistry to measure the interaction between molecules. Autofluorescence refers to the natural fluorescence emitted by certain molecules in tissues, so it is not relevant to detecting antinuclear antibodies.

The Standard Method for Detecting Antinuclear Antibodies (In Certain Autoimmune