In DNA sequencing,what is the purpose of the dideoxynucleotide bases?

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The purpose of dideoxynucleotide bases is to terminate the growth of a DNA chain at a specific point during the process of DNA sequencing. This is because dideoxynucleotides lack the 3'-OH group required for the formation of a phosphodiester bond, which is essential for chain elongation. As a result, when incorporated into a growing DNA chain, a dideoxynucleotide will terminate the chain, resulting in a series of fragments of varying lengths that can be separated and read to determine the sequence of the original DNA template.

In DNA Sequencing,what Is the Purpose of the Dideoxynucleotide Bases