In a polymerase chain reaction,the threshold cycle represents:

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The threshold cycle (Ct) in a polymerase chain reaction (PCR) represents the cycle at which the fluorescence signal of the amplified product crosses a certain threshold above background level. This threshold is typically set at a level where there is a reliable detection and quantification of the target DNA. The Ct value is inversely proportional to the amount of target DNA in the original sample, so a higher Ct value indicates a lower amount of starting material, while a lower Ct value indicates a higher amount of starting material. Therefore, the Ct value is an indication of the original amount of target in the sample.

In a Polymerase Chain Reaction,the Threshold Cycle Represents