In flow cytometry,it is possible to detect the expression of 10 different cell membrane proteins simultaneously by:

Question

Quizplus · Accepted Answer

Staining the cells with 10 different labeled probes that fluoresce at different wavelengths is the most feasible and common method to detect the expression of multiple cell membrane proteins simultaneously in flow cytometry. Each labeled probe will bind specifically to its target protein, and the fluorescence produced by the probes can be detected by specific detectors, which allows the identification of different cell populations based on their protein expression profiles. Using 10 different lasers or measuring light scattering at different angles may not provide enough specificity and sensitivity to detect multiple proteins at the same time, while measuring mass besides fluorescent intensity is not a common practice in flow cytometry.

In Flow Cytometry,it Is Possible to Detect the Expression of 10