Defects in oxidative burst activity can be detected using flow cytometry by labeling patient neutrophils with:

Question

Quizplus · Accepted Answer

Dihydrorhodamine (DHR) is a fluorescent probe that is used to measure oxidative burst activity in neutrophils. When DHR enters the cell, it is oxidized by reactive oxygen species (ROS) generated during the oxidative burst, resulting in fluorescence. Flow cytometry can be used to measure the amount of fluorescence in individual neutrophils, allowing for detection of defects in oxidative burst activity. Nitroblue tetrazolium (NBT) is also commonly used to assess oxidative burst activity, but requires an additional step of staining the cells with the dye after activation. Purified protein derivative (PPD) and phytohemagglutinin (PHA) are not relevant for measuring oxidative burst activity.

Defects in Oxidative Burst Activity Can Be Detected Using Flow