A toxicology screen is set up using thin layer chromatographic plates.Four positive controls/standards are used with the following distance of migration from application point results: Std A = 8 cm; Std B = 15 cm; Std C = 22.The acetone solvent front moved 35 cm.After processing an unknown sample,a solute had a migration distance of 16 cm.What is the R_f of the unknown solute?

Question

Quizplus · Accepted Answer

R_f can be calculated using the following formula: R_f = (distance migrated by the solute) / (distance migrated by the solvent front) For the unknown solute, Distance migrated by the solute = 16 cm Distance migrated by the solvent front = 35 cm Therefore, R_f = 16/35 = 0.45 The best choice is B as it is the only option that matches the value calculated using the formula.

A Toxicology Screen Is Set Up Using Thin Layer Chromatographic