In partition chromatography,separation is based on:

Question

Quizplus · Accepted Answer

Partition chromatography separates components based on their relative solubility in the stationary and mobile phases. The stationary phase is usually coated with a hydrophobic material that selectively binds to certain compounds. The mobile phase is a polar solvent, such as water or alcohol. As the mixture travels through the column, components with higher solubility in the stationary phase will move more slowly and be retained longer than those with lower solubility. This results in a separation of the mixture into its individual components. Choice A is incorrect because hydrogen bonding and hydrophobic interactions are more commonly seen in other types of chromatography, such as reversed-phase chromatography. Choice B is incorrect because partition chromatography uses a stationary phase with small pores to increase surface area and increase binding capacity. Choice C is incorrect because this describes affinity chromatography, which uses specific molecules immobilized on the matrix to capture target molecules in the sample.

In Partition Chromatography,separation Is Based On