In clinical applications using gas chromatography,which one of the following column temperature techniques will cause the more volatile analytes to elute first and therefore produce the best separation of complex mixtures of analytes with a range of volatilities?

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Temperature programmed operation involves gradually increasing the column temperature over time, which causes the more volatile analytes to elute first and the less volatile analytes to elute later. This technique produces the best separation of complex mixtures of analytes with a range of volatilities. Isothermal operation and room temperature operation do not provide enough temperature variation to effectively separate analytes with different volatilities. Refrigerator temperature operation is too low and may cause condensation of some analytes, leading to poor resolution.

In Clinical Applications Using Gas Chromatography,which One of the Following