In a continuous-monitoring assessment of an enzyme reaction rate,which one of the following is the preferable measurement?

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Quizplus · Accepted Answer

In a continuous-monitoring assessment of an enzyme reaction rate, the preferable measurement is to measure the increasing concentration of product, as it directly reflects the rate of the enzyme-catalyzed reaction. Measurement of the decreasing concentration of substrate may not be as accurate, as the initial concentration of substrate may not be homogeneous throughout the reaction mixture or may be affected by non-enzymatic reactions. Direct measurement of enzyme protein may not indicate the rate of the reaction, as the concentration of the enzyme alone does not indicate how fast the reaction is proceeding. Calculation of K_m and V_max may be useful for determining the Michaelis-Menten constant and maximum reaction velocity, respectively, but they do not provide continuous monitoring of the reaction rate.

In a Continuous-Monitoring Assessment of an Enzyme Reaction Rate,which One