The reference method for protein determination that converts nitrogen to ammonium ions by acid digestion is the:

Question

Quizplus · Accepted Answer

The Kjeldahl method involves the digestion of the protein sample with concentrated sulfuric acid, which results in the conversion of nitrogen to ammonium ions. The ammonium ions are then liberated by the addition of an alkaline solution and are measured by titration or colorimetric methods. The biuret method, absorption at 280 nm, and refractometry method do not involve digestion and are not as reliable for accurately determining protein concentration.

The Reference Method for Protein Determination That Converts Nitrogen to Ammonium