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Why Do Values Obtained with the Jaffe Reactions for Creatinine

Question 3

Multiple Choice

Why do values obtained with the Jaffe reactions for creatinine measurement overestimate creatinine concentration by ~20%?


A) The components of the reaction mixture often continue to react at endpoint and synthesize excess creatinine.
B) The rate of Jaffe complex formation is temperature dependent,and excess heat formed by the reaction leads to increased absorptivity and falsely increased creatinine value.
C) Noncreatinine chromogens in the reaction that react with the picrate in the end-point Jaffe methods overestimate true plasma creatinine concentration.
D) Hydroxide concentrations above 0.5 mmol/L produce a decreased degradation of the Jaffe complex.

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