The oxidation of the Fe⁺² of hemoglobin to the Fe⁺³ of methemoglobin by ferricyanide is the initial step in the:

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The oxidation of Fe⁺² to Fe⁺³ by ferricyanide is a crucial step in the cyanmethemoglobin method of hemoglobin determination. This method involves the conversion of hemoglobin to cyanmethemoglobin, which is red and has a well-defined absorption spectrum. The amount of cyanmethemoglobin formed is directly proportional to the hemoglobin concentration in the sample, which can be measured spectrophotometrically. The Mentzer index reaction is used for screening of F1F1F1S1F1F1F10-thalassemia, the Ehrlich reaction is used for the determination of bilirubin, and HPLC methods are used for separating hemoglobin fractions. None of these methods involves the oxidation of Fe⁺² to Fe⁺³ by ferricyanide.

The Oxidation of the Fe+2 of Hemoglobin to the Fe+3 α\alphaα

The Oxidation of the Fe⁺² of Hemoglobin to the Fe⁺³ $\alpha$