How does a dideoxynucleotide (ddNTP)that is used in DNA sequencing methods differ from a typical deoxynucleotide (dNTP)present in normal DNA?

Question

Quizplus · Accepted Answer

A ddNTP lacks a hydroxyl group at the 3' carbon, which prevents further elongation of the DNA strand during sequencing. This allows for specific termination and identification of each nucleotide in the sequence. A typical dNTP does have a hydroxyl group at the 3' carbon, allowing for continued polymerization of the DNA strand.

How Does a Dideoxynucleotide (DdNTP)that Is Used in DNA Sequencing