In a pyrosequencing reaction,the incorporation of a nucleotide releases _____.Eventually,visible light is produced by an enzyme reaction.

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In pyrosequencing, the incorporation of a nucleotide releases pyrophosphate, which is then detected by ATP sulfurylase and converted to ATP. The ATP then reacts with luciferin in the presence of luciferase, resulting in the production of visible light. A primer is required for initiating the sequencing reaction, but it is not directly involved in the release of pyrophosphate or the production of light.

In a Pyrosequencing Reaction,the Incorporation of a Nucleotide Releases _____