Which PCR step causes the denaturation of double-stranded DNA?
A) Add DNA polymerase and nucleotides at 72°C.
B) Cool DNA to between 50°C and 65°C.
C) Add primers.
D) Heat target DNA to 94°C.
E) Repeat the cycle of heating and cooling.
Correct Answer:
Verified
Q10: DNA polymerases used in PCR
A)use an RNA
Q11: The deliberate removal of genetic material from
Q12: DNA strands can be clipped crosswise at
Q13: EcoRI and HindIII are
A)palindromes.
B)reverse transcriptases.
C)restriction endonucleases.
D)ligases.
E)DNA polymerases.
Q14: The fluorescent in situ hybridization (FISH) probes
Q16: Which PCR step synthesizes complementary DNA strands?
A)Add
Q17: Labeled, known, short stretches of DNA used
Q18: Gene probes can be labeled for detection
Q19: Amplification of DNA is accomplished by
A)polymerase chain
Q20: The function of the dideoxy (dd) nucleotides
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