When determining the sequence of nucleotides in an unknown sample of DNA, which method is used to sequence the DNA?

Question

Quizplus · Accepted Answer

Sanger sequencing is the most common method used to determine the sequence of nucleotides in DNA. It is a type of chain termination sequencing that involves sequencing fragments of the DNA by using dideoxynucleotides that terminate the elongation of the DNA chain. PCR amplification is often used to generate enough DNA for sequencing, but it is not a sequencing method itself. Cloning and Southern blot hybridization are also not sequencing methods.

When Determining the Sequence of Nucleotides in an Unknown Sample