Before a desired gene can be spliced into a bacterial plasmid cloning vector,the gene must first be ___ from the original DNA.

Question

Quizplus · Accepted Answer

Before a gene can be spliced into a cloning vector, it must be precisely cut with a restriction enzyme. This creates "sticky ends" that can then be matched with complementary sticky ends on the plasmid vector, allowing the gene to be inserted into the vector. Option A is incorrect because injecting the gene into a different organism would not make sense for creating a cloning vector. Option B is incorrect because cloning refers to the process of making multiple copies of a specific DNA sequence, not extracting it from a mix of recipient DNA. Option C is incorrect because stem cells are not typically used in the process of creating a cloning vector.

Before a Desired Gene Can Be Spliced into a Bacterial