What enzyme is used to "glue" together the ends of cut pieces of DNA?

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme used to catalyze the formation of covalent bonds between the sugar-phosphate backbone of two pieces of DNA, forming a phosphodiester bond that "glues" them together. This is a key step in many genetic engineering techniques, including creating recombinant DNA molecules and cloning. DNA polymerase is also involved in DNA synthesis but is not used to specifically join two separate pieces of DNA. RNA polymerase is responsible for transcription, not DNA ligation. Restriction enzymes are used to cut DNA at specific sites, not to join them. Protease cleaves proteins and is not directly involved in DNA ligation.

What Enzyme Is Used to "Glue" Together the Ends of Cut