Place the following steps for the cloning of a gene in order: Place the following steps for the cloning of a gene in order:
1)DNA ligase seals foreign DNA into a plasmid.
2)A restriction enzyme is used to cleave DNA.
3)The bacterium makes a product.
4)Some of the bacterial cells take up a recombinant plasmid.
5)The plasmid replicates on its own.
A)2,1,4,5,3
B)1,2,3,4,5
C)3,1,2,4,5
D)5,1,4,3,2
E)1,3,2,4,5

Question

Quizplus · Accepted Answer

First, a restriction enzyme cleaves the DNA (2), then DNA ligase seals the foreign DNA into a plasmid (1). Some bacterial cells take up the recombinant plasmid (4), the plasmid replicates (5), and finally, the bacterium makes a product (3).

Place the Following Steps for the Cloning of a Gene