Given the following peptide, what pair of protease enzymes would be able to produce three smaller peptides? Gly-Ser-Ala-His-Phe-Pro-Asn-Ala-Val-Glu-Cys-Ala-Ser
A)chymotrypsin and thrombin
B)trypsin and chymotrypsin
C)thermolysin and chymotrypsin
D)trypsin and thermolysin
E)none of the above

Question

Quizplus · Accepted Answer

Thermolysin cleaves at hydrophobic residues, and chymotrypsin cleaves at aromatic residues. Gly-Ser-Ala-His-Phe-Pro-Asn-Ala-Val-Glu-Cys-Ala-Ser has four aromatic residues (Phe and His) and four hydrophobic residues (Val, Ala, and two Cys) which can be cleaved by thermolysin and chymotrypsin, respectively. Therefore, using thermolysin and chymotrypsin together would produce three smaller peptides.

Given the Following Peptide, What Pair of Protease Enzymes Would