What role does the coenzyme A portion of acetyl-CoA play in the reaction catalyzed by citrate synthase?
A)the CoA group causes the enzyme to close around the substrates, enabling catalysis
B)lack of resonance in the thioester makes the methyl carbon of the acetyl group more acidic, allowing proton abstraction to readily occur
C)CoA makes the carbonyl carbon highly susceptible to nucleophilic attack
D)hydrolysis of the thioester makes the reaction essentially irreversible
E)all of the above

Question

Quizplus · Accepted Answer

The coenzyme A portion of acetyl-CoA plays multiple roles in the reaction catalyzed by citrate synthase: it causes the enzyme to close around the substrates, enabling catalysis; the lack of resonance in the thioester bond makes the methyl carbon more acidic, facilitating proton abstraction; it makes the carbonyl carbon highly susceptible to nucleophilic attack; and the hydrolysis of the thioester bond makes the reaction essentially irreversible.

What Role Does the Coenzyme a Portion of Acetyl-CoA Play