TRIS {(HOCH₂)₃CNH₂} is one of the most common buffers used in biochemistry.A solution is prepared by adding enough TRIS and 12 M HCl(aq)to give 1.00 L of solution with [TRIS] = 0.30 M and [TRISH⁺] = 0.60 M.What is the pH of this buffered system if the pK_b is 5.92? A)5.92 B)6.22 C)7.78 D)8.08

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Quizplus · Accepted Answer

The pH of the buffer can be calculated using the Henderson-Hasselbalch equation for a base: pH = 14 - pKb + log([base]/[acid]). Here, [base] = 0.30 M and [acid] = 0.60 M, and pKb = 5.92. So, pH = 14 - 5.92 + log(0.30/0.60) = 7.78.

TRIS {(HOCH2)3CNH2} Is One of the Most Common Buffers Used

TRIS {(HOCH₂)₃CNH₂} Is One of the Most Common Buffers Used