One of the assumptions of the Hardy-Weinberg theorem is that

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One of the assumptions of the Hardy-Weinberg theorem is that mutations must not occur or a mutational equilibrium must exist. This means that the frequency of new mutations should be low enough to not significantly affect the gene frequencies in the population over time. The other options listed are not assumptions of the theorem. Mating within the population is assumed to be random, the size of the population can be any size, gene frequencies may change due to natural selection or other factors, and migration into and out of the population can occur but should not significantly affect gene frequencies either.

One of the Assumptions of the Hardy-Weinberg Theorem Is That