You are purifying a nuclease by affinity chromatography.To determine which fractions contain the protein of interest,you test samples of all fractions for their ability to break down DNA.This is an example of
A)a binding assay.
B)a biological assay.
C)an enzyme assay.
D)an immunological assay.
E)none of the above

Question

Quizplus · Accepted Answer

This is an example of an enzyme assay because the test measures the ability of the nuclease (an enzyme) to catalyze the breakdown of DNA, which is the enzymatic activity of interest.

You Are Purifying a Nuclease by Affinity Chromatography