The pK₁,pK₂,and pK_R of the amino acid histdine are 1.8,9.3,and 6.0,respectively.The pK₁,pK₂,and pK_R of the amino acid arginine are 1.8,9.0,and 12.5,respectively.You have a mixture of histidine and arginine,how would you try to separate these two amino acids?

Question

Quizplus · Accepted Answer

At pH 9, histidine will be negatively charged (deprotonated) and will bind to the positively charged anion exchange resin, while arginine will be positively charged (protonated) and will not bind to the resin. This will allow the two amino acids to be separated.

The PK1,pK2,and PKR of the Amino Acid Histdine Are 1

The PK₁,pK₂,and PK_R of the Amino Acid Histdine Are 1