DNA fragments are usually inserted into a plasmid vector and cloned before they are analyzed by chain termination sequencing because

Question

Quizplus · Accepted Answer

A) DNA fragments need to be pure in order to obtain an unambiguous sequence. Cloning and amplification in a plasmid vector ensures purity of the DNA fragments.
B) A large number of identical fragments is needed in a sequencing reaction in order to generate detectable amounts of products. Cloning and amplification in a plasmid vector results in multiple copies of the DNA fragment.
C) Plasmid vectors have known sequences that can be used for design and annealing of the primer oligonucleotides, which are used to initiate the sequencing reaction. This makes it easier to design primers specific to the DNA fragment of interest.

DNA Fragments Are Usually Inserted into a Plasmid Vector and Cloned