I propose to design a new drug which will act as an inhibitor for an enzyme.If I have used all current information about the mechanism of this enzyme to design this inhibitor and I carefully engineer it with similar chemical properties of the transition state,what type of inhibitor am I attempting to engineer and how will I know if I have succeeded? A)A competitive inhibitor,collect kinetic data both in the presence and absence of inhibitor and watch for a change in V_max. B)A competitive inhibitor,collect kinetic data both in the presence and absence of inhibitor and watch for a change in K_M. C)A uncompetitive inhibitor,collect kinetic data both in the presence and absence of inhibitor and watch for a change in K_M. D)A uncompetitive inhibitor,collect kinetic data both in the presence and absence of inhibitor and watch for a change in V_max. E)None of the above.

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Quizplus · Accepted Answer

A competitive inhibitor binds to the active site of an enzyme and prevents the substrate from binding. If the inhibitor has similar chemical properties to the transition state, it will bind very tightly to the enzyme and be a potent inhibitor. To know if you have succeeded, you can collect kinetic data both in the presence and absence of inhibitor and watch for a change in Vmax. If Vmax decreases in the presence of inhibitor, then you have successfully engineered a competitive inhibitor.

I Propose to Design a New Drug Which Will Act