Which of the following would NOT result in the relaxation of supercoiled DNA?
A)heating the DNA to induce denaturation
B)nicking one strand
C)treatment with pancreatic DNaseI
D)treatment with type II topoisomerase
E)treatment with type I topoisomerase

Question

Quizplus · Accepted Answer

Heating the DNA to induce denaturation would result in the separation of the double strands and the formation of single-stranded DNA, but it would not necessarily relax the supercoiling of the DNA. The other options all have the potential to relax supercoiled DNA in different ways.

Which of the Following Would NOT Result in the Relaxation