The sequence-specific binding of BamHI to DNA depends primarily on
A)hydrogen bonding and allows binding to less specific sequences as well.
B)hydrogen bonding and precludes binding to any other sequence.
C)stacking interactions and could be mimicked using an intercalation molecule.
D)hydrogen bonding and could be mimicked using an intercalation molecule.
E)none of the above

Question

Quizplus · Accepted Answer

The sequence-specific binding of BamHI to DNA precludes binding to any other sequence. This is because the enzyme recognizes and binds to a specific short sequence of six nucleotides known as the recognition sequence or restriction site. This binding is mediated by hydrogen bonding between specific amino acid residues in the enzyme and the nucleotide bases in the DNA. Therefore, choice A is incorrect as it suggests that BamHI can bind to less specific sequences. Choice C and D are incorrect as stacking interactions and intercalation molecules are not involved in the sequence-specific binding of BamHI to DNA.

The Sequence-Specific Binding of BamHI to DNA Depends Primarily on