Which enzyme is used to join two complementary DNA fragments after a cut has been made?
A) restriction endocnucleases
B) RNA polymerase
C) DNA gyrase
D) DNA ligase
E) Helicase

Question

Quizplus · Accepted Answer

DNA ligase is the enzyme responsible for joining two complementary DNA fragments after a cut has been made. This enzyme catalyzes the formation of phosphodiester bonds between the 3'-hydroxyl of one nucleotide and the 5'-phosphate of another nucleotide in the presence of ATP. This results in the formation of a continuous strand of DNA.

Which Enzyme Is Used to Join Two Complementary DNA Fragments