Why is it possible to join a piece of DNA from a human that has been digested using EcoR1 and a piece of DNA from a bacterium that has been digested using EcoR1?
A) They have been cut with the same restriction enzyme therefore the staggered ends are the same and are complementary
B) Bacteria and humans exist in a symbiotic relationship and therefore share DNA and can be joined together
C) They can join together only in the presence of high heat and pressure, such as is found in an autoclave.
D) They can not join together because the DNA has come from two different species and even though they are digested with the same restriction enzyme the cuts are at different locations.
E) All of these

Question

Quizplus · Accepted Answer

The fact that both DNA samples have been cut using the same restriction enzyme (EcoR1) means that they will have the same sticky ends, which are complementary and can therefore be joined together by ligase. B, C, and D are incorrect statements.

Why Is It Possible to Join a Piece of DNA