The purpose of the primer sequence in PCR is to:
A) Provide a promoter sequence for DNA polymerase
B) Provide a free 3'-OH end required for covalent extension
C) Allow for efficient denaturation of the double stranded DNA
D) Keep the DNA denatured after the temperature is reduced
E) All of these

Question

Quizplus · Accepted Answer

The primer sequence in PCR is designed to provide a free 3'-OH end which is essential for covalent extension by DNA polymerase. This allows the polymerase to add nucleotides to the end of the primer, creating a complementary sequence to the template DNA. Choices A, C and D are incorrect as they do not accurately describe the function of the primer sequence in PCR.

The Purpose of the Primer Sequence in PCR Is To