A physical map of a chromosome can be created by:
A) Mapping the restriction cleavage sites in the chromosome
B) Using electron microscopy
C) Using western blotting
D) Using recombination experiments
E) All of these

Question

Quizplus · Accepted Answer

Mapping the restriction cleavage sites in the chromosome allows for the identification and location of specific DNA sequences on the chromosome, which can then be used to create a physical map. Electron microscopy can be used to visualize the chromosome, but does not provide specific location information. Western blotting is a technique used to detect specific proteins and would not be useful in creating a physical map of a chromosome. Recombination experiments can provide information on the relative location of genes on a chromosome, but may not give a complete picture of the physical structure of the chromosome.

A Physical Map of a Chromosome Can Be Created By