Which of the following techniques is used to test for unstable trinucleotide repeats associated with Huntington's disease?
A) Positional cloning
B) DNA sequencing
C) PCR
D) In situ hybridization
E) Northern blotting

Question

Quizplus · Accepted Answer

PCR is the most commonly used method to detect unstable trinucleotide repeats associated with Huntington's disease. Specifically, PCR amplifies the DNA region containing the trinucleotide repeat, and the length of the amplified product indicates the number of repeats present. In Huntington's disease, the number of CAG repeats in the huntingtin gene is expanded in affected individuals.

Which of the Following Techniques Is Used to Test for Unstable