During a polymerase chain reaction,
A) a DNA template is mixed with primers, nucleotides, and Taq polymerase.
B) the sample is heated in order to allow the two strands of DNA to separate completely.
C) some of the primers base-pair with template DNA.
D) Taq polymerase performs DNA synthesis from the primers.
E) all of these occur.

Question

Quizplus · Accepted Answer

All of these steps occur during a polymerase chain reaction. A DNA template is mixed with primers, nucleotides, and Taq polymerase. The sample is heated to allow the two strands of DNA to separate completely. Some of the primers base-pair with the template DNA, and Taq polymerase performs DNA synthesis from the primers.

During a Polymerase Chain Reaction