An Hfr strain of E.coli with the genotype gly⁺azi^RStr^S is mated with an F^-strain of E.coli of genotype gly^-azi^SStr^R.Gly refers to the amino acid glycine,azi refers to sodium azide,and Str refers to the antibiotic streptomycin.Conjugation occurs and the progeny are screened on a selective medium to detect recombinants.If you wanted to select for the F^- recombinant genotype gly⁺azi^RStr^R,you should use a minimal medium containing:

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The medium should contain streptomycin and sodium azide to select for the F- recombinants that are resistant to both, while not requiring glycine since the desired recombinants can synthesize it.

An Hfr Strain of E