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A Mixed Infection of an E

Question 41

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A mixed infection of an E.coli strain was performed using the h-r+ and h+r- genotypes of the T2 phage.Progeny phage were collected after lysis and plated on two E.coli strains to obtain plaque characters important for detection of parental and recombinant types.
h+ can only infect one strain of E.coli
h- can infect both strains
r+ shows slower lysis,forms small plaques
r- shows rapid lysis
In the table below,for each observed phenotype,give the genotype that would produce that phenotype,and indicate if the phenotype is parental or recombinant type.Calculate the map distance between the h and r genes using the information provided.
 Plaque phenotype  Number of plaques  Plaque genotype  Parental/recombinant  cloudy, large 600 clear, small 480 cloudy, small 180 clear, large 180\begin{array} { | l | l | l | l | } \hline \text { Plaque phenotype } & \text { Number of plaques } & \text { Plaque genotype } & \text { Parental/recombinant } \\\hline \text { cloudy, large } & 600 & & \\\hline \text { clear, small } & 480 & & \\\hline \text { cloudy, small } & 180 & & \\\hline \text { clear, large } & 180 & & \\\hline\end{array}

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RF% = (recombinants/total)blured image 100 = map dis...

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