A plasmid vector has a gene for erythromycin resistance (Ery^R)and a gene for ampicillin resistance (Amp^R).The Amp gene is cut with restriction enzyme,and donor DNA treated with the same enzyme is added.What genotype of cells needs to be selected to show evidence of transformation? A)Amp^REry^R B)Amp^REry^S C)Amp^SEry^R D)Amp^SEry^S E)None of the answer options are correct.

Question

Quizplus · Accepted Answer

To show evidence of transformation, cells must have taken up the plasmid with the donor DNA incorporated. Since the Amp gene was cut and donor DNA was added, successful transformants would have the donor DNA inserted at the Amp site, disrupting the Amp resistance gene. Therefore, cells that are resistant to erythromycin (indicating they have the plasmid) but sensitive to ampicillin (indicating the Amp gene was disrupted) need to be selected. This is represented by the genotype in option C, where "R" indicates resistance and "S" indicates sensitivity.

A Plasmid Vector Has a Gene for Erythromycin Resistance (EryR)and

A Plasmid Vector Has a Gene for Erythromycin Resistance (Ery^R)and