Which is the synthetic inducer Jacob and Monod used to examine the expression of $\beta$-galactosidase?
A)allolactose
B)isopropyl-$\beta$-D-thiogalactoside
C)lactose
D)albumin
E)galactose

Question

Quizplus · Accepted Answer

Jacob and Monod used isopropyl-β-D-thiogalactoside (IPTG) as a synthetic inducer to examine the expression of lac operon in E. coli. This is because IPTG is a non-hydrolyzable analog of allolactose, which is the natural inducer of the lac operon. Lactose and galactose cannot be used as synthetic inducers because they do not efficiently penetrate the cell membrane and also get metabolized by other enzymes. Albumin is a protein and not a synthetic inducer.

Which Is the Synthetic Inducer Jacob and Monod Used to Examine