Pyrosequencing determines the nucleotide sequence because:
A)dideoxy nucleotides are incorporated into a growing DNA strand.
B)dideoxy nucleotides terminate the growing DNA strand.
C)PPi is incorporated into the growing DNA strand.
D)PPi is released from the growing DNA strand.
E)None of the answer options are correct.

Question

Quizplus · Accepted Answer

Pyrosequencing relies on the detection of pyrophosphate (PPi) released upon the incorporation of a nucleotide into the DNA strand being synthesized. This release of PPi is then used in a series of enzymatic reactions that ultimately produce a detectable light signal, indicating which nucleotide was incorporated.

Pyrosequencing Determines the Nucleotide Sequence Because